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Project Topic:

THE ANTIBACTERIAL ACTIVITY OF AZADARICHTA INDICA (NEEM PLANT)

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 Format: MS WORD ::   Chapters: 1-5 ::   Pages: 30 ::   Attributes: Data Analysis,abstract, table of content, references ::   107 people found this useful

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ABSTRACT

 

The antibacterial effect of Azadirachta indica (neem plant) against Pseudomonas aeruginosa, Klebsiella ozanae, Staphylococcus aureus and Escherichia coli was determined using the agar cup plate techniques. The phytochemical components of A. indica showed the presence of saponin and phlobatanin and the absence of alkaloids, tannis, phenolics, plycosides, flavonoids, and triterpenes, the result showed that the test organisms were susceptible to 500mg/ml, 50mg/ml and 5mg/ml of the plant extract. The minmum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined, the result showed that the MIC and MBC were 5mg and 50mg respectively for Pseudomonas aeruginosa, K. ozanae, Staphylococcus aureus and Escherichia coli. The result of the study suggest that extracts of A. indica could be suitable for the treatment of various infections caused by P. aeruginosa, K. ozanae, S. aureus and E. coli.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

TABLE OF CONTENT

Title page                                                                                           i

Certification                                                                                      ii

Dedication                                                                                         iii

Acknowledgement                                                                             iv

Abstract                                                                                             v

Table of Content                                                                                vi-vii

CHAPTER ONE

 

  1. Introduction                                                                            1-2
    1. Literature review                                                                      3-7CHAPTER TWO

 

  1. Materials and methods                                                            8
    1. Collection and preparation of samples                                     8
    2. Collection of specimen                                                             8
    3. Extraction of materials                                                             8
    4. Phytochemical screening of extracts of A. indica                      8
    5. Anti-microbial susceptibility test                                             9
    6. Determination of minimum inhibitory concentration (MIC)     9CHAPTER THREE

 

  1. Result                                                                                      10
  2. Phytochemical activities of the extracts                                    10
  3. Antimicrobial activities of the extracts                                     10-12CHAPTER FOUR4.0          Discussion                                                                               13-14

 

          CHAPTER FIVE

5.0     Conclusion                                                                              15

References                                                                               16

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CHAPTER ONE

 

 

 

1.0    INTRODUCTION

 

 

 

Plants contain many biologically active compounds which have potential for development as medicinal agents. Herbal medicines already form the basis of therapeutic use in the developing countries but on recent there has been an increase in the use of herbal medicine in the developed world too (De and Ifeoma, 2002; El-manhood et al, 2010) plants provide alternative strategy in search for new drug. There is a rich abundance of plant reported in traditional medicine to process protective and therapeutic properties (Kayode and Kayode, 2011). It is likely that plants will continue to be a valuable sources of new molecules which may, after possible  chemical manipulation, provide new and improve drugs (Shah et al; 2006) Bacterial resistance to antibiotic represents a serious problem for clinicians and the pharmaceutical industry and great efforts are being made to reverse this trend and one of them is the widespread screening of medicinal plant from the traditional system to medicine hoping to get some newer, saver and more effective agents that can be used to fight infectious diseases (Natarian et al, 2003)

 

Azandirachta indica is one of such medical plants belonging to the meliaceae family and the indigenous to Southern Asia (Akula et al, 2003) Azadirachta indica commonly known as neem has attracted worldwide prominence in recent years, owing to its wide range of medicinal properties Neem has been extensively used in Ayurveda Umani and homoeopathic medicine and has become a cynosure of modern medicine

 

Neem elaborates a fast array of biologically active compound that are chemically diverse and structurally complex.

 

More than 140 compounds have been isolated from different parts of neem. All parts of the neem tree leaves, flowers, seeds, fruits and roots and sac bark have been used traditionally for the treatment of inflammations infections, fever, skin diseases and dental disorders. The medicinal utilities have been described especially for neem leaf. Neem leaf and its constituents have been demonstrated to exhibit immunomodulatory anti-Inflammatory,  antihyperglycaemic, Antiurcer, antimalaria, antifungal, antibacterial, antiviral, antiocidant, antimutagenic and anticarcinogenic properties (Talwar et al;1997 Biswas et al 2002; Subapriya and Nagine; 2005).

 

The objective of this study therefore are to determine the phytochemical components of the leaf extract of A. Indica to determine the minimum  inhibitory concentration (MIC) of the extract on Pseudomonas aeruginosa, Nebsiella Ozanas, Staphylococcus aureus and Escherichia coli.


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Paper Information

Format:ms word
Chapter:1-5
Pages:30
Attribute:Data Analysis,abstract, table of content, references
Price:₦3,000
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